Science

Hemp Science:

Cannabidiolic Acid-Synthase / CBDA-Synthase (CBDAS) is the end of Hemp’s Cannabinoid gene pathway.

Fresh Hemp Greens and Hemp Microgreens generally contain at best 0.0006, or 6 parts per 10,000 (0.20 X 0.003) at harvest.  Dried these Hemp Herbs can contain up to 0.003 by definition and are still considered Hemp.

gpp-pathway-no-cbd-only-cbdas


Cannabis Science:

Tetra-hydrocannabinolic Acid-Synthase / THCA-Synthase (THCAS) is the end of Cannabis’ Cannabinoid gene pathway.

Fresh Cannabis Greens and Cannabis Microgreens generally contain up to 0.02%, or 2 parts per 1,000 at harvest.  Dried these Cannabis Herbs can contain up to 2.0%, or 2 parts per 100 in the first 30 days of curing, and up to 8.0%, or 8 part per 100 upon complete curing.

gpp-pathway-no-thc-only-thcas


The Biosynthesis of Cannabis – a little more detail

In Fig. 1 you will have seen the names and acronyms of a number of compunds, which probably say nothing about the actual pathway! So I’ll try to decribe it more clearly here.

As shown in Fig. 1, there are two compounds whose expression (the amount of them that is produced in the leaf) is controlled by a specific area of the Cannabis plantgenome called locus A. These two compounds acts as the “core” upon which other things are added, later on in the pathway. Divarinic acid is identical to olivetolic acid, except for a hydrocarbon chain coming off the phenolic ring, which in the latter is slightly longer:

As the carboxyl group (-COOH) and a hydroxyl group (-OH) are found on neighbouring carbons, decarboxylation can occur upon heating in the absence of enzyme. Therefore, the pathway shown in Fig. 1 assumes that decarboxylation occurs before the addition of geranyl pyrophosphate. Fig. 3 shows one of the routes through the pathway, using the skeletal structures of the compounds involved:

Source:http://2015.igem.org/Team:Oxford/Blog


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